To determin the related substances in the raw material of berberine hydrochloride by HPLC.A Spherigel C18 chromatographic column(150 mm×4.6 mm,5μm)was used with a mobile phase consisting of 0.02 mol∙L^-1ammonium acetate-acetonitrile solution(70∶30)at the flow rate of 0.6 mL∙min^-1.The detection wavelength was 265 nm，the temperature was 30 ℃,the injection was 10 μL and the detector was ultraviolet detector(UVD).The results showed that under the optimum conditions,the berberine hydrochloride and its related substances could be separated well（R>1.5）.Berberine hydrochloride and its related substances showed a good linear relationship(R²>0.99).The established method is sensitive，effective，reproducible and easy to handle，which can be used for the determinating of the related substances in berberine hydrochloride.

The Contents of Two Triterpenes in Rosa Laevigata Michx. were Determined by HPLC [J]. Journal of Shenyang University of Chemical Technology, 2021, 35(3): 218-222.