Curcumin nano-suspension thermosensitive hydrogel was prepared with blank gel prepared by Poloxamer 407 (P407),Poloxamer 188 (P188) and carboxymethyl chitosan (CMC) as matrix,combined with curcumin nano-suspension prepared by anti-solvent method.The formulation and preparation process of curcumin nano-suspension were optimized by star point design-response surface method.Taking the phase transition temperatures t1 and t2 of gel before and after being diluted by simulated tears as dependent variables.The fitting equation,variance analysis and comparison of predicted and actual values were used to find the best process formula for the preparation of temperature sensitive hydrogels.FT-IR,SEM,XRD，UV-Vis and dialysis bag method were used to evaluate the success of preparation and in vitro performance.The results showed that when the mass fraction ratio of P407 to P188 was 5∶1,the volume fraction of CMC was 14.5%,and the volume fraction of GA was 3%,the deviation between the predicted and measured gelation temperature of the prepared thermosensitive hydrogel was within ± 1%,and it showed a dynamic flow at room temperature,and it was 33 ℃ after diluted with simulated tears,which meets the basic requirements of gelling.This research provides a new idea for the clinical application of the preparation.